Cells were stained with anti-IgE FITC in that case, anti-CD4 PERCP, anti-B220 PERCP and anti-CD200R PE for 30 min in 4 C, washed twice with 2 mL of PBS and centrifuged in 500for 5 min

Cells were stained with anti-IgE FITC in that case, anti-CD4 PERCP, anti-B220 PERCP and anti-CD200R PE for 30 min in 4 C, washed twice with 2 mL of PBS and centrifuged in 500for 5 min. positive marker and a combined mix of anti-B220-PERCP and anti-CD4-PERCP as adverse markers led to a well-separated basophil population. Extra … Continue reading Cells were stained with anti-IgE FITC in that case, anti-CD4 PERCP, anti-B220 PERCP and anti-CD200R PE for 30 min in 4 C, washed twice with 2 mL of PBS and centrifuged in 500for 5 min